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Molecular Detection of Human Bacterial Pathogens

Molecular Detection of Human Bacterial Pathogens PDF Author: Dongyou Liu
Publisher: CRC Press
ISBN: 1439812381
Category : Medical
Languages : en
Pages : 1278
Book Description
As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses this issue, with international scientists in respective bacterial pathogen research and diagnosis providing expert summaries on current diagnostic approaches for major human bacterial pathogens. Each chapter consists of a brief review on the classification, epidemiology, clinical features, and diagnosis of an important pathogenic bacterial genus, an outline of clinical sample collection and preparation procedures, a selection of representative stepwise molecular protocols, and a discussion on further research requirements relating to improved diagnosis. This book represents a reliable and convenient reference on molecular detection and identification of major human bacterial pathogens; an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in bacterial characterization; and an essential textbook for undergraduate and graduate students in microbiology.

Molecular Detection of Human Bacterial Pathogens

Molecular Detection of Human Bacterial Pathogens PDF Author: Dongyou Liu
Publisher: CRC Press
ISBN: 1439812381
Category : Medical
Languages : en
Pages : 1278
Book Description
As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses this issue, with international scientists in respective bacterial pathogen research and diagnosis providing expert summaries on current diagnostic approaches for major human bacterial pathogens. Each chapter consists of a brief review on the classification, epidemiology, clinical features, and diagnosis of an important pathogenic bacterial genus, an outline of clinical sample collection and preparation procedures, a selection of representative stepwise molecular protocols, and a discussion on further research requirements relating to improved diagnosis. This book represents a reliable and convenient reference on molecular detection and identification of major human bacterial pathogens; an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in bacterial characterization; and an essential textbook for undergraduate and graduate students in microbiology.

Molecular Detection of Human Viral Pathogens

Molecular Detection of Human Viral Pathogens PDF Author: Dongyou Liu
Publisher: CRC Press
ISBN: 9781138115170
Category :
Languages : en
Pages : 1180
Book Description
Despite being recognized and fought against over countless centuries, human viral pathogens continue to cause major public health problems worldwide--killing millions of people and costing billions of dollars in medical care and lost productivity each year. With contributions from specialists in their respective areas of viral pathogen research, Molecular Detection of Human Viral Pathogensprovides a reliable reference on molecular detection and identification of major human viral pathogens. Each chapter briefly reviews the classification, epidemiology, clinical features, and diagnosis of one related viral pathogen or a group of them. The clinical sample collection and preparation procedures are outlined, and a selection of representative stepwise molecular detection protocols is covered. The chapters conclude with a discussion on further research requirements relating to improved diagnosis. With its judicious selection of streamlined, ready-to-use protocols for major human viral pathogens--including commercial kits--Molecular Detection of Human Viral Pathogensis an indispensable tool for medical, veterinary, and industrial laboratory scientists involved in virus determination.

Molecular Detection of Foodborne Pathogens

Molecular Detection of Foodborne Pathogens PDF Author: Dongyou Liu
Publisher: CRC Press
ISBN: 9781420076448
Category : Science
Languages : en
Pages : 905
Book Description
While the vast majority of our food supplies are nutritious and safe, foodborne pathogen-related illness still affects millions of people each year. Large outbreaks of foodborne diseases- such as the recent salmonella outbreak linked to various peanut butter products- continue to be reported with alarming frequency.All-Encompassing Guide to Detecti

Molecular Food Microbiology

Molecular Food Microbiology PDF Author: Dongyou Liu
Publisher: CRC Press
ISBN: 1351120360
Category : Science
Languages : en
Pages : 464
Book Description
The elucidation of DNA double helix in 1953 and the publication of DNA cloning protocol in 1973 have put wings under the sail of molecular biology, which has since quietly revolutionized many fields of biological science, including food microbiology. Exploiting the power and versatility of molecular technologies, molecular food microbiology extends and greatly improves on phenotypically based food microbiology, leading to the development of better diagnostics for foodborne infections and intoxications, and contributing to the design of more effective therapeutics and prophylaxes against foodborne diseases. Forming part of the Food Microbiology series, Molecular Food Microbiology provides a state of art coverage on molecular techniques applicable to food microbiology. While the introductory chapter contains an overview on the principles of current DNA, RNA and protein techniques and discusses their utility in helping solve practical problems that food microbiology is facing now and in the future, the remaining chapters present detailed moleuclar analyses of selective foodborne viruses, bacteria, fungi and parasites. Key Features: Contains a state of art overview on moleuclar techniques applicable to food microbiology research and development Presents in-depth molecular analysis of selective foodborne viruses, bacteria, fungi and parasites Highlights the utility of molecular tecniques for accurate diagnosis and effective control of foodborne diseases Includes expert contributions from international scientists involved in molecular food microbiology research Represents a highly informative textbook for students majoring in food, medical, and veterinary microbiology Offers a contemporary reference for scholars and educators wishing to keep abreast with the latest developments in molecular food microbiology With contributions from international scientists involved in molecular food microbiology research, this book constitutes an informative textbook for undergraduates and postgraduates majoring in food, medical, and veterinary microbiology; represents an indispensable guide for food, medical, and veterinary scientists engaged in molecular food microbiology research and development; and offers a contemporary update for scholars and educators trying to keep in touch with the latest developments in molecular food microbiology.

Molecular Medical Microbiology

Molecular Medical Microbiology PDF Author: Yi-Wei Tang
Publisher: Academic Press
ISBN: 0323899927
Category : Science
Languages : en
Pages : 3506
Book Description
Molecular Medical Microbiology was the first book to synthesise the many new developments in both molecular and clinical research in a single comprehensive resource. The molecular age has brought about dramatic changes in medical microbiology, and great leaps in our understanding of the mechanisms of infectious disease. This 3rd edition of Molecular Medical Microbiolog 3rd , completely updated, reviewed, and expanded with an extra volume will again be the must-have on every shelf of every hospital lab and university department. Molecular Medical Microbiology, 3rd is a very timely and helpful update for microbiologists, students and clinicians in the ear of increasing use of molecular techniques, changing epidemiology and prevalence and increasing resistance of many pathogenic bacteria. Written by experts in the field, chapters include cutting edge information, and clinical overviews for each major bacterial group, in addition to the latest updates on vaccine development, molecular technology and diagnostic technology and expanded with an extra 4th volume dedicated to virology. Completely updated, revised comprehensive and accessible reference on molecular medical microbiology Full color presentation throughout In-depth discussion of individual pathogenic bacteria in a system-oriented approach Includes a clinical overview for each major bacterial group Presents the latest information on vaccine development, molecular technology, and diagnostic technology More than 100 chapters covering all major groups of bacteria Written by an international panel of authors who are experts in their respective disciplines

Molecular Detection of Zoonotic Bacteria

Molecular Detection of Zoonotic Bacteria PDF Author: Subir Sarker
Publisher: LAP Lambert Academic Publishing
ISBN: 9783659172687
Category :
Languages : en
Pages : 80
Book Description
It is worthy to consider the live bird markets are important for the existence of avian reservoir bacteria that are enteric human pathogens. With this ignition, need arises to determine the occurrence of zoonotic bacteria from the workers of live bird markets. The interaction of humans with poultry provides a considerable risk of revelation to bacteria. Although some of these markets have been as potential sources of zoonotic bacterial disease and molecular detection positivity among live bird market workers in Bangladesh raises anew the considerable risks associated with these practices. Accordingly, the results obtained in this study suggest that the appropriate precautions should be taken during and subsequent to the handling of live birds to minimize the risk of zoonotic diseases.

Molecular Food Microbiology

Molecular Food Microbiology PDF Author: Dongyou Liu
Publisher: CRC Press
ISBN: 1351120379
Category : Science
Languages : en
Pages : 464
Book Description
The elucidation of DNA double helix in 1953 and the publication of DNA cloning protocol in 1973 have put wings under the sail of molecular biology, which has since quietly revolutionized many fields of biological science, including food microbiology. Exploiting the power and versatility of molecular technologies, molecular food microbiology extends and greatly improves on phenotypically based food microbiology, leading to the development of better diagnostics for foodborne infections and intoxications, and contributing to the design of more effective therapeutics and prophylaxes against foodborne diseases. Forming part of the Food Microbiology series, Molecular Food Microbiology provides a state of art coverage on molecular techniques applicable to food microbiology. While the introductory chapter contains an overview on the principles of current DNA, RNA and protein techniques and discusses their utility in helping solve practical problems that food microbiology is facing now and in the future, the remaining chapters present detailed moleuclar analyses of selective foodborne viruses, bacteria, fungi and parasites. Key Features: Contains a state of art overview on moleuclar techniques applicable to food microbiology research and development Presents in-depth molecular analysis of selective foodborne viruses, bacteria, fungi and parasites Highlights the utility of molecular tecniques for accurate diagnosis and effective control of foodborne diseases Includes expert contributions from international scientists involved in molecular food microbiology research Represents a highly informative textbook for students majoring in food, medical, and veterinary microbiology Offers a contemporary reference for scholars and educators wishing to keep abreast with the latest developments in molecular food microbiology With contributions from international scientists involved in molecular food microbiology research, this book constitutes an informative textbook for undergraduates and postgraduates majoring in food, medical, and veterinary microbiology; represents an indispensable guide for food, medical, and veterinary scientists engaged in molecular food microbiology research and development; and offers a contemporary update for scholars and educators trying to keep in touch with the latest developments in molecular food microbiology.

Diagnostic Bacteriology Protocols

Diagnostic Bacteriology Protocols PDF Author: Louise O’Connor
Publisher: Springer Science & Business Media
ISBN: 1597451436
Category : Science
Languages : en
Pages : 226
Book Description
The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.

Laboratory Models for Foodborne Infections

Laboratory Models for Foodborne Infections PDF Author: Dongyou Liu
Publisher: CRC Press
ISBN: 1351646397
Category : Medical
Languages : en
Pages : 832
Book Description
Resulting from ingestion of inappropriately prepared or stored foods containing pathogenic viruses, bacteria, fungi and parasites, foodborne infections have become a significant source of human morbidity and mortality worldwide in recent decades. This may be largely attributable to the remarkable popularity of convenient, ready-to-eat food products, the dramatic expansion of international food trades, and the continuing growth of immuno-suppressed population groups. Although anti-microbial treatments have played a crucial part in the control of foodborne infections in the past, the emergence and spread of anti-microbial resistance render the existing treatments ineffective. Additionally, our limited understanding of the molecular mechanisms of foodborne infections has thwarted our efforts in the development of efficacious vaccines for foodborne pathogens. Given the obvious benefits of laboratory models in foodborne disease research, a great number of experiments have been conducted toward the elucidation of host-pathogen interactions in and pathogenic mechanisms of foodborne infections. Forming part of the Food Microbiology series, Laboratory Models for Foodborne Infections presents a state-of-the-art review of laboratory models that have proven valuable in deciphering the life cycle, epidemiology, immunobiology, and other key aspects of foodborne pathogens. Written by scientists with respective expertise in foodborne pathogen research, each chapter includes a contemporary summary of a particular foodborne viral, bacterial, fungal, or parasitic infection in relation to its life cycle, epidemiology, clinical features, pathogenesis, host-pathogen interactions, and other related aspects. Besides providing a trustworthy source of information for undergraduates and postgraduates in food microbiology, Laboratory Models for Foodborne Infections offers an invaluable guide for scientists and food microbiologists with interest in exploiting laboratory models for detailed study of foodborne infections.

Detection of Zoonotic Bacterial Pathogens in Multiple Hosts Using a Microbiome Approach and Molecular Characterization of Anaplasma Phagocytophilum

Detection of Zoonotic Bacterial Pathogens in Multiple Hosts Using a Microbiome Approach and Molecular Characterization of Anaplasma Phagocytophilum PDF Author: Agatha Onyemowo Kolo
Publisher:
ISBN:
Category : Anaplasma
Languages : en
Pages : 376
Book Description
The Mnisi community, a rural area, is nestled at the cusp of a human/livestock/wildlife interface in Bushbuckridge Municipality, Mpumalanga Province, South Africa. In this area, undifferentiated non-malarial acute febrile illness (AFI) is among the most common presenting signs in patients seeking healthcare at the community clinics. Recent research suggested that zoonotic pathogens either rodent-borne or tick-borne may be common aetiologies of febrile illness in the community. The study had shown that patients presenting with non-malarial AFI had prior exposure to Bartonella spp., spotted fever group Rickettsia, Coxiella burnetii and Leptospira spp. Low levels of West Nile and Sindbis, but no Rift Valley fever virus exposure were found. In a separate study, the molecular detection of a bacterium closely related to Anaplasma phagocytophilum in dog samples collected in the Mnisi community was also reported. The aim of this study was, therefore to investigate wild rodents, cattle and dogs as well as their associated ticks, as possible sources of zoonotic pathogen infection in the Mnisi community using a microbiome sequencing approach. We also screened AFI patient samples, rodents, dogs, cattle and ticks for the presence of A. phagocytophilum using a real-time PCR assay. The Anaplasma species detected were subsequently characterized using gene sequencing and phylogenetic analysis. The sample set consisted of 282 wild rodents trapped across three habitat types, 74 AFI patients, 56 domestic dogs, 100 cattle, 160 Rhipicephalus sanguineus ticks collected from dogs and 348 Amblyomma hebraeum ticks collected from cattle. Of these, the bacterial blood microbiome of a subset of samples was generated using circular consensus sequencing (CCS) performed on the Pacific Biosciences platform at Washington State University. The full sample set was then also screened for the specific presence of A. phagocytophilum using a Taqman real-time PCR assay, followed by the molecular characterization and phylogenetic analysis of A. phagocytophilum targeting the 16S rRNA, gltA, ankA and msp4 genes. The bacterial blood microbiome of 25 Mastomys rodent species collected from three habitat areas revealed a total of 65,060 bacterial sequences with 29% of the total sequence reads obtained corresponding to Bartonella grahamii, 23% to Bartonella sp. strain RF255YX and 12% of sequences to Bartonella spp. Overall, rodents from Hlalakahle (urban/periurban area) and Tlhavekisa (communal rangeland) had higher proportions of Bartonella species (~85%), while Gottenburg (urban/periurban area) and Manyeleti (protected area) had lower Bartonella spp. loads (~45%). Other organisms of zoonotic and veterinary significance detected included Ehrlichia sp. (~0.03%), C. burnetii (~0.02%), Anaplasma spp. (~0.5%), and Brucella spp. (~1%). Characterization of the blood microbiome of the dogs revealed 30,340 bacterial sequences, with 24% of the total sequences obtained from canine blood corresponding to Ehrlichia canis, 19.3% to A. platys, 14.8% to Anaplasma sp. ZAM dog, while 0.3% of sequences corresponded to A. phagocytophilum. The characterization of the blood microbiome from nine cattle revealed 34,559 bacterial sequences, of which 58% corresponded to A. marginale, 22.2% to Anaplasma sp. Mymensingh, 10.5% to Anaplasma spp. and 5.4% to Anaplasma sp. Dedessa. In addition, these species were detected in the following rates in cattle blood: Anaplasma sp. Hadesa: 2.7%, A. centrale: 1.4%, Bartonella spp.: 0.5%, A. platys: 0.2%, Anaplasma sp. Saso: 0.2% and A. phagocytophilum: 0.01%. Characterization of the bacterial microbiome from 24 pools of salivary glands and 23 pools of midgut tissues from the 348 A. hebraeum ticks produced a total of 86,308 bacterial sequence reads from the midgut pools as well as 84,420 sequences from the salivary gland pools. Of these, 81.7% of the bacterial sequences from the midgut pools and 83% of the sequences from the salivary gland pools were dominated by the zoonotic pathogen Rickettsia africae, the cause of African tick bite fever (ATBF). A further 6.8% of the sequences from the salivary gland pools and 6.9% of the sequences from the midgut pools corresponded to Rickettsia spp. Six percent of the total sequences from the salivary gland pools and 3.4% of the sequences from the midgut pools corresponded to E. ruminantium, while 1.4% of the sequence reads from the salivary gland pools and 1.2% of the reads from the midgut pools corresponded to Coxiella spp. symbionts. Characterization of the blood microbiome of nine AFI patients revealed 13,726 bacterial sequences. Of significance was the detection of R. africae from three AFI patients and Brucella melitensis from one AFI patient. DNA from 74 non-malarial acute febrile illness patients, 282 rodents, 100 cattle, 56 dogs, and 160 R. sanguineus ticks were screened using a quantitative real-time PCR designed to target the msp2 gene of A. phagocytophilum. However, the test was found to detect A. phagocytophilum and an Anaplasma sp. recently described from Zambian dogs (Anaplasma sp. ZAM dog). Sequencing of the 16S rRNA and gltA genes confirmed the presence of A. phagocytophilum DNA in humans, dogs and rodents; also highlighting its potential importance as a possible contributing cause of acute febrile illness in humans in this rural community in South Africa. Anaplasma sp. ZAM dog and Anaplasma platys were furthermore identified in dogs, while Candidatus Anaplasma boleense and Anaplasma sp. Mymensingh were identified in cattle. Anaplasma sp. ZAM dog was also identified in R. sanguineus ticks collected from dogs. Phylogenetic analyses grouped Anaplasma sp. ZAM dog into a distinct clade; providing sufficient divergence with the other Anaplasma species to warrant classification as a separate species. Until appropriate type-material can be deposited and the species can be formally described, we will refer to this novel organism as Anaplasma sp. SA dog (for Anaplasma sp. southern Africa dog). In conclusion, the detection of an array of zoonotic bacterial pathogens from wild rodents, domestic dogs, cattle and their associated ticks and humans in this study highlights their significance as possible contributing factors to non-malarial febrile illness in the Mnisi community area. We therefore recommend that healthcare practitioners in the community should consider them in the differential diagnosis of AFI.